Overview Microbiological Management Environmental Management Microbiological Testing
Overview
Microbiological Management
Environmental Management
Microbiological Testing
Overview
Purpose
The purpose is to periodically assess the health of laboratory animals so that their quality is suitable for the research objectives, to minimize the impact of environmental factors on the animals and thereby derive reliable and reproducible experimental results, and to respond promptly when abnormalities occur in the animals' husbandry environment or health condition, minimizing the effect on experimental outcomes.
Classification
Microbiological management of laboratory animals
Environmental management of the laboratory animal facility
Microbiological testing of drinking water, feed, and bedding
Microbiological Management of Laboratory Animals
Operating Principles
As a rule, testing is conducted regularly once per quarter per room, for a total of 4 times per year, and may be conducted irregularly when disease occurs in the laboratory animals.
Rodents, Guinea pigs, and Rabbits are tested regularly, while medium and large animals are tested for specified items during acceptance inspection and quarantine.
When animals are introduced from a production facility not under contract with Seoul National University Hospital or from an external research facility, an SPF certification must be attached.
Even if an SPF certification is provided, additional testing is conducted when the tested items are insufficient, in which case the researcher pays the fee.
Test Items, Classification, Testing Cycle, and Method
Mouse
Mouse microbiological test items
Category Class Pathogen Testing Cycle Test Method SPF Re-entry
Virus A Hantavirus ○ ○ ELISA A Lymphocytic choriomeningitis virus (LCMV) ○ ○ B Mouse hepatitis virus (MHV) ○ ○ C Murine norovirus (MNV) ○ ○ B Sendai virus (HVJ) ○ ○ C Theiler's murine encephalomyelitis virus (GDⅦ, TMEV) ○ - B Ectromelia virus (Mousepox) ○6 ○6 C Minute virus of mice (MVM) ○6 - C Mouse adenovirus (MAV) ○6 - C Mouse rotavirus (EDIM) ○6 - C Pneumonia virus of mice (PVM) ○6 - C Reovirus type 3 ○6 - C K virus ○12 - C Lactate dehydrogenase-elevating virus (LDEV) ○12 - C Mouse cytomegalovirus (MCMV) ○12 - C Mouse parvovirus (MPV) ○12 - C Mouse thymic virus (MTV) ○12 - IFA C Polyoma virus ○12 - ELISA Bacteria C Bordetella bronchiseptica ○ ○ Cultrue C Citrobacter rodentium ○ ○ C Corynebacterium bovis ○ ○ C Corynebacterium kutscheri ○ ○ C Helicobacter bilis ○ ○ PCR C Helicobacter hepaticus ○ ○ C Helicobacter typhlonius ○ ○ D Klebsiella oxytoca ○ ○ Cultrue D Klebsiella pneumoniae ○ ○ B Mycoplasma pulmonis ○ ○ ELISA C Rodentibacter pneumotropicuc ○ ○ Cultrue C Rodentibacter heylii ○ ○ D Pseudomonas aeruginosa ○ ○ A Salmonella spp. ○ ○ D Staphylococcus aureus ○ ○ D β-haemolytic Streptococci ○ ○ D Streptococcus pneumoniae ○ ○ C Cilia-associated respiratory (CAR) bacillus ○6 ○6 ELISA C Clostridium piliforme (Tyzzer's disease) ○6 ○6 A Streptobacillus moniliformis ○12 ○12 ELISA Parasites C Ectoparasites ○ ○ Microscopy C Giardia spp, Spironucleus spp. ○ ○ E Helminths (incl. Pinworms) ○ ○ E Nonpathogenic protozoa (incl. Entamoeba spp, Hexamita muris, Trichomonas) ○ ○ Fungi D Pneumocystis murina ○ ○ PCR A Dermatophytes ○6 ○6 Cultrue(Stain) A Encephalitozoon cuniculi ○12 ○12 ELISA
Rat
Rat microbiological test items
Category Class Pathogen Testing Cycle Test Method SPF Re-entry
Virus A Hantavirus ○ ○ ELISA C Rat coronavirus (Sialodacryoadenitis virus, SDAV) ○ ○ B Sendai virus (HVJ) ○ ○ C H-1 virus ○6 - C Kilham rat virus (KRV) ○6 - C Mouse adenovirus (MAV) ○6 - C Pneumonia virus of mice (PVM) ○6 - C Rat minute virus (RMV) ○6 - C Rat parvovirus (RPV) ○6 - C Reovirus type 3 ○6 - C Rat theilovirus (RTV) ○12 - Bacteria C Bordetella bronchiseptica ○ ○ Cultrue C Corynebacterium kutscheri ○ ○ C Helicobacter bilis ○ ○ PCR D Klebsiella oxytoca ○ ○ Cultrue D Klebsiella pneumoniae ○ ○ B Mycoplasma pulmonis ○ ○ ELISA C Rodentibacter pneumotropicuc ○ ○ Cultrue C Rodentibacter heylii ○ ○ D Pseudomonas aeruginosa ○ ○ A Salmonella spp. ○ ○ D Staphylococcus aureus ○ ○ D β-haemolytic Streptococci ○ ○ D Streptococcus pneumoniae ○ ○ C Cilia-associated respiratory (CAR) bacillus ○6 ○6 ELISA C Clostridium piliforme (Tyzzer's disease) ○6 ○6 A Streptobacillus moniliformis ○12 ○12 Parasites C Ectoparasites ○ ○ Microscopy C Giardia spp, Spironucleus spp. ○ ○ E Helminths (incl. Pinworms) ○ ○ E Nonpathogenic protozoa (incl. Entamoeba spp, Hexamita muris, Trichomonas) ○ ○ Fungi D Pneumocystis carinii ○ ○ PCR A Dermatophytes ○6 ○6 Cultrue(Stain) A Encephalitozoon cuniculi ○12 ○12 ELISA
Guinea Pig
Guinea pig microbiological test items
Category Class Pathogen Testing Cycle (SPF) Test Method
Virus A Lymphocytic choriomeningitis virus (LCMV) ○ ELISA C Sendai virus ○ C Guinea pig adenovirus ○6 Bacteria C Bordetella bronchiseptica ○ Cultrue C Pasteurella multocida ○ C Pasteurella pneumotropica ○ A Salmonella spp. ○ C β-haemolytic Streptococci ○ C Streptococcus pneumoniae ○ C Clostridium piliforme (Tyzzer's disease) ○ ELISA A Streptobacillus moniliformis ○12 Parasites E Ectoparasites ○ Microscopy C Eimeria spp. ○ Fungi A Dermatophytes ○ Cultrue(Stain) A Encephalitozoon cuniculi ○6 ELISA
Rabbit
Rabbit microbiological test items
Category Class Pathogen Testing Cycle (SPF) Test Method
Virus B Rabbit rotavirus ○ ELISA C Sendai virus ○ C Rabbit pox virus (Myxomatosis) ○6 Bacteria C Bordetella bronchiseptica ○ Cultrue B Pasteurella multocida ○ A Salmonella spp. ○ B Clostridium piliforme (Tyzzer's disease) ○ ELISA Parasites E Ectoparasites ○ Microscopy C Eimeria spp. ○ Fungi A Dermatophytes ○ Cultrue(Stain) A Encephalitozoon cuniculi ○ ELISA
Medium & Large Animals
Visual inspection and administration of antiparasitic agents upon introduction
Parasite testing and microbiological testing when disease occurs
Primates
Regular health monitoring is conducted
Physical examination, CBC, parasite testing, tuberculosis testing, microbiological testing (viruses, bacteria)
Department of Experimental Animal Research Monitoring Reports
Environmental Management of the Laboratory Animal Facility
Recommended Environmental Control Values
Recommended environmental control values by animal species
Rodents Rabbit/Guinea Pig Medium·Large/Primates Marmoset Animal Room Other Areas Animal Room Other Areas Animal Room Other Areas Animal Room Other Areas
Temperature 20-26 16-22 18-29 24-29 Humidity 30-70 30-70 30-70 30-70 30-70 30-70 30-70 30-70 Illuminance 150-300 300~600 150-300 300~600 800-1100 300~600 800-1100 300~600 Noise 60 or below 60 or below 60 or below 60 or below Ammonia 20 or below - 20 or below - 20 or below - 20 or below - Settled Bacteria 30 3 100 3 100 3 100 3 Differential Pressure Positive Positive Negative Positive
Quality Control of Drinking Water, Bedding, and Feed
Drinking Water Quality Control
Internal Quality Testing
pH (5.8~8.5)
Viable cell count (100 or fewer)
Growth of Pseudomonas aeruginosa
Regular testing: once every 3 months
Irregular testing: when water contamination is suspected
External Quality Testing
Apply the drinking water test items and standards of the Seoul Metropolitan Government Research Institute of Public Health and Environment
Regular testing: once every 6 months
Irregular testing: when water contamination is suspected
Feed & Bedding Microbiological Testing
Feed & Bedding Testing
Cycle: once every 6 months
Total bacteria · Coliforms
Salmonella · Fungi
