Equipment

High Content Screening System - Operetta CLS

High Content Screening System Operetta CLS specifications

Model	Operetta CLS
Manufacturer	Revvity (PerkinElmer)
Year Introduced	2017
Usage Type	Analysis Request (paid)
Features	Spinning disk type confocal Automated imaging based on multi-well (96/348) plates (laser autofocusing) → analysis of characteristics at the individual cell level → quantification (phenotype classification) Machine learning (PhenoLOGIC ML-based analysis) → coding-free analysis via the built-in analysis software → segmentation, phenotype classification, cell population, feature extraction, 3D culture, spheroid, organoid, and cell-surface texture analysis (image based) Multi-imaging modality Fluorescence, brightfield, digital phase contrast (DPC), and confocal (spinning disk) imaging available Temperature and CO2 control available for long-term imaging
Usage	Reservation required 3 days before use → equipment warming up Use of PhenoPlate (standard) recommended
Location	Medical Research Innovation Center (CMI), Room B2213

Real-time Cell Metabolism Analyzer - Seahorse XFe96

Real-time Cell Metabolism Analyzer Seahorse XFe96 specifications

Model	Seahorse XFe96
Manufacturer	Agilent
Year Introduced	2020
Usage Type	Analysis Request (paid)
Features	Real-time kinetic analysis of mitochondrial respiration and cellular glycolysis via OCR/ECAR measurement Label-free, non-invasive measurement (maintaining the live-cell state) 96-well high-throughput (drug screening) Automated drug injection system
Usage	Reservation required 1 week before use → equipment warming up; preparation of sensor plate and HCO3- free media; a cell pretreatment plan is required (prior consultation needed)
Location	Medical Research Innovation Center (CMI), Room B2213

Exosome Characterization System - ExoView R100

Exosome Characterization System ExoView R100 specifications

Model	ExoView R100
Manufacturer	Unchained Labs
Year Introduced	2021
Usage Type	Analysis Request (paid)
Features	Particle size analysis (label-free method using interferometric imaging) Exosome marker analysis (CD9 / CD63 / CD81 / exosome cargo via fluorescence labeling) Single EV analysis → single-vesicle-level analysis rather than bulk EV analysis → heterogeneity analysis possible Antibody-based capture (microarray chip) → direct analysis without EV purification (plasma, urine, CSF, cell culture media, etc.) Multi-parameter analysis → particle count, size distribution, surface marker, co-localization, EV phenotype profiling Applications: Cancer liquid biopsy, EV biomarker, drug delivery vesicle, immunology EV profiling
Usage	Reservation required 2 weeks before use → preparation time needed for dedicated microchip, buffer, washing solution, conjugated antibodies, and sample Having prior extracellular vesicle counting data obtained with an NTA instrument helps optimize the sample loading amount
Location	Medical Research Innovation Center (CMI), Room B2213

Real-time Cell Imaging System - MuviCyte

Real-time Cell Imaging System MuviCyte specifications

Model	MuviCyte
Manufacturer	Revvity (PerkinElmer)
Year Introduced	2022
Usage Type	Analysis Request (paid)
Features	A microscope installed inside an incubator (CO2/O2 adjustable) → minimizes cell stress / enables long-term observation Long-term time-lapse imaging → records the entire process of cellular change (proliferation/apoptosis kinetics analysis) Equipped with a 96-well wound scratcher (wound healing/migration) Drug response kinetics Cytotoxic assay Spheroid growth Stem cell tracking Transfection efficiency
Usage	Reservation required 3 days before use → equipment warming up, CO2/O2 condition adjustment When using a 96-well wound maker, use of SPL products is recommended Fluorescence phototoxicity (repeated imaging at short intervals → induces cell stress → minimize exposure / imaging interval adjustment needed. Phototoxicity: GFP Imaging interval: Proliferation → 30~60 min Migration → 5~15 min
Location	Medical Research Innovation Center (CMI), Room B2213